| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06712SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC8529G-025 | |||
| Carrier Free | IC8529R-025 | |||
| Carrier Free | HAM85291 | |||
| Carrier Free | IC8529V-100UG | |||
| Carrier Free | IC8529T-100UG | |||
| Carrier Free | IC8529U-100UG | |||
| Carrier Free | IC8529S-100UG | |||
| Carrier Free | IC8529N-100UG | |||
| Carrier Free | IC8529R-100 | |||
| Carrier Free | IC8529G-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37823-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08994-0.025mg | |||
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged protein demonstrating that the rat monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0010.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged protein demonstrating that the rat monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged SFO-p41 protein with DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712]. Observed molecular weight ~46.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0005.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged AIFm2 protein (A), DYKDDDDK tagged IREB2 protein (B), and vector control (C) using DYKDDDDK epitope tag antibody (L5) [NBP1-06712] at 2 ug/ml.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of HeLa cells transfected with a FLAG encoded construct (top) or untransfected (bottom), using DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712]. Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Immunocytochemistry-Immunofluorescence-NBP1-06712-img0009.jpg)
![Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), and FLAG (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32901033/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Immunohistochemistry-NBP1-06712-img0011.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Detection of DDK in SUSD3 overexpression lysate using [NBP1-06712] followed by goat anti-rat IgG HRP conjugated secondary antibody (cat.# NB7115). Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Rat Monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis using DyLight 488 conjugated antibody (NBP1-06712G). Normal mouse prostate cells overexpressed with Flag-tagged construct (Green: Flag). Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-06712_rat-monoclonal-dykddddk-epitope-tag-antibody-l5-immunocytochemistry-immunofluorescence-792023163813..jpg)
![Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Generation of a wrapping glia driver.Confocal projection of larval filet preparations of the genotypes indicated. Representative images are shown taken from >10 animals analyzed for each genotype. a Third instar larva with the genotype [nrv2-stGFP]. Broad GFP expression is detected in the CNS. Note the restricted expression in the peripheral nervous system which corresponds to the wrapping glia (arrowheads). b Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), & FLAG (blue). c Same animal as in (a). Expression of stRed is observed only in the CNS & no expression is found in the wrapping glia. d–f Overlay of nrv2-GFP (green) & 90C03 > dsRed (red) expression. Note the complete overlap of dsRed (e) & GFP expression in the CNS (f). g Young third instar larva with the genotype [hs-Flp; nrv2-Gal4/MCFO-2; 90C03-Gal80]. flp expression was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), & FLAG (blue). h Living third instar larva of the genotype [nrv2-Gal4, UAS-CD8GFP; 90C03-Gal80/90C03-Gal80]. Note strong expression at the anterior tip of the larva. Scale bars are 250 µm (a–c, g, h) & 100 µm (d–f). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32901033), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06712_rat-monoclonal-dykddddk-epitope-tag-antibody-l5-310202416182637.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-344-img0009.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa.")
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Analysis using the Agarose Immobilized conjugate of DYKDDDDK Epitope Tag Antibody [NB600-344]. DYKDDDK in transfected COS-7 cells using agarose immobilized DYKDDDDK Epitope Tag Antibody.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-344-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI and a PLA against the FLAG epitope (DYKDDDDK Epitope Tag Antibody [NB600-344]) was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122059), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-344-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-344-img0011.jpg)
![Proximity Ligation Assay: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI & a PLA against the FLAG epitope was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25909512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-344_goat-dykddddk-epitope-tag-pab-bsa-free-31020241535645.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - used for Western Blot. Lane 1: Untransfected HEK (control) Lane 2: Transfected HEK (FLAG-tagged protein, clone 1) Lane 3: Transfected HEK (FLAG-tagged protein, clone 2). Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-345-img0008.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - used for Western Blot. Lane 1: Untransfected HEK (control) Lane 2: Transfected HEK (FLAG-tagged protein, clone 1) Lane 3: Transfected HEK (FLAG-tagged protein, clone 2). Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-345] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-345] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-345-img0007.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-345] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-345-img0002.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Use of DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] in Western Blot. Flag expression is significantly increased in 293 cells transfected with a flag-fusion protein. Image from a verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-29E4-G7-Western-Blot-NBP1-97410-img0009.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Use of DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] in Western Blot. Flag expression is significantly increased in 293 cells transfected with a flag-fusion protein. Image from a verified customer review.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Twenty-four clones were randomly selected and grown up from glycerol stocks by inoculating 0.5 mL 2X YT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 uL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410].](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-29E4-G7-Western-Blot-NBP1-97410-img0004.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Antibody detects both C-terminal linked and N-terminal linked FLAG tagged recombinant proteins.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-29E4-G7-Western-Blot-NBP1-97410-img0011.jpg)
![ELISA: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Detection of DYKDDDDK conjugated proteins using the DyLight 405 conjugated version of DYKDDDDK Epitope Tag Antibody (29E4.G7).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-29E4-G7-ELISA-NBP1-97410-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. Fusion nuclear protein (red) stained with purified DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] (detection by Goat anti-mouse IgG1 Alexa Fluor 594).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0004.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. Fusion nuclear protein (red) stained with purified DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] (detection by Goat anti-mouse IgG1 Alexa Fluor 594).")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] stained with DAPI (blue).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554]. Merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0003.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis using [NBP2-37823] at a 1:1000 (1ug/mL) dilution, probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Observed molecular weight ~130 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Western-Blot-NBP2-37823-img0011.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis using [NBP2-37823] at a 1:1000 (1ug/mL) dilution, probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Observed molecular weight ~130 kDa.")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA (blue) in HeLa cells. Cells were infected with a GFP-expressing strain of Chlamydia trachomatis containing FLAG-IncA under the control of the Tetracycline (Tet) promoter, and either left untreated (0ng/ml Tet) or treated with anhydrotetracycline (10ng/ml Tet). Cells were fixed with 2% paraformaldehyde in PBS for 30 min at RT, permeabilized and blocked in 10% goat serum, 0.1% BSA, 0.1% saponin in PBS pH 7.4 for 30 mins at RT, then probed with a DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] at 1:500 for 1 hr at RT. Cells were washed with PBS, and incubated with an AlexaFluor 647-conjugated goat anti-mouse IgG secondary antibody at 1:2000 for 1 hr at RT. Images were taken on a fluorescent microscope using a 60X oil-immersion objective. Scale bars represent 20um. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Immunocytochemistry-Immunofluorescence-NBP2-37823-img0009.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of 30ug of whole cell lysates from HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under the control of a Tetracycline promoter (Tet), and either left untreated (-) or treated with anhydrotetracycline (+), onto an SDS-PAGE gel, using [NBP2-37823]. Observed molecular weight ~30 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Western-Blot-NBP2-37823-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA in HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under control of a Tetracycline (Tet) promoter, untreated (-) or treated with anhydrotetracycline (+). Complexes formed by incubating 1ml of HeLa cell lysate with 3ug of [NBP2-37823] overnight at 4C. Immune complexes were captured on 20ul Protein G agarose and eluted by boiling in Laemmli sample buffer. Samples were resolved on a 10% Bis-Tris acrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hr at RT. Membrane was probed with a rabbit anti-FLAG pAb at 1:200 in 3% BSA in TBST overnight at 4C, washed in TBST, and incubated with an HRP-conjugated donkey anti-rabbit IgG secondary Ab at 1:15000 in 1% milk in TBST for 1 hr at RT. Chemiluminescence performed using SuperSignal West Dura. Data courtesy of Innovators Program.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Immunocytochemistry-Immunofluorescence-NBP2-37823-img0010.jpg)
![Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] - Analysis of 750 ug whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 ug whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 ug of DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DYKDDDDK Epitope Tag Antibody (231) diluted at 1 : 5000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-231-Immunoprecipitation-NBP2-43570-img0001.jpg "Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] - Analysis of 750 ug whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 ug whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 ug of DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DYKDDDDK Epitope Tag Antibody (231) diluted at 1 : 5000. EasyBlot anti-mouse IgG was used as a secondary reagent.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] - DDDDK Tag antibody [GT231] detects DDDDK Tag protein by Western blot analysis.A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of DDDDK-tagged protein expressing 293T cells12 % SDS-PAGEDDDDK Tag antibody [GT231] (NBP2-43570) dilution: 1:5000](http://images.novusbio.com/fullsize/nbp2-43570_mouse-dykddddk-epitope-tag-mab-231-9102024205644.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody [NBP2-37833] - Analysis of recombinant protein containing the epitope tag sequence DYKDDDDK using DYKDDDDK Epitope Tag Antibody [NBP2-37833].](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NBP2-37833-img0002.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NBP2-37833] - Analysis of recombinant protein containing the epitope tag sequence DYKDDDDK using DYKDDDDK Epitope Tag Antibody [NBP2-37833].")
![Western Blot: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] - Analysis of 40 ug whole cell extracts from HeLa cells transfected with either Ty1-tagged MBD1 (lanes 1 and 3) or Ty1-tagged MBD2 (lane 2 and 4). The WB was performed with the DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] diluted 1:1000 in TBS-Tween containing 5% skimmed milk (lane 1 and 2) or with an MBD1 antibody (lane 3) or an MBD2 antibody (lane 4). The position of the Ty1-tagged MBD1 and Ty1-tagged MBD2 are shown on the right, the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-C-15200054-Western-Blot-NBP2-59146-img0004.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] - Analysis of 40 ug whole cell extracts from HeLa cells transfected with either Ty1-tagged MBD1 (lanes 1 and 3) or Ty1-tagged MBD2 (lane 2 and 4). The WB was performed with the DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] diluted 1:1000 in TBS-Tween containing 5% skimmed milk (lane 1 and 2) or with an MBD1 antibody (lane 3) or an MBD2 antibody (lane 4). The position of the Ty1-tagged MBD1 and Ty1-tagged MBD2 are shown on the right, the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] - Results obtained with the [NBP2-59146]. ChIP assays were performed using Plasmodium falciparium cells stably expressing Ty1-H2A (blue bars) and wild type Plasmodium falciparium cells (brown bars). Primer pairs were used for active genes, inactive genes, and variable genes. Sheared chromatin from 1x10^6 cells and 2 ug of antibody against Ty1 were used per ChIP experiment.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-C-15200054-Chromatin-Immunoprecipitation-NBP2-59146-img0002.jpg)
![ELISA: DYKDDDDK Epitope Tag Antibody (M2.1) - Chimeric [NBP3-08994] - ELISA using M2.1 and Anti-i-motif DNA [iMab]. Binding curves of the rabbit IgG chimeric version of the Anti-DYKDDDDK Epitope Tag antibody M2.1 (NBP3-08994; blue line) and isotype control (NBP2-52638, anti-fluorescein; orange line) to an ELISA plate coated with Anti-i-motif DNA [iMab] at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Anti-i-motif DNA [iMab] antibody. For signal detection, a 1:4000 dilution of a Goat Anti-Rabbit IgG antibody was used.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-M2-1-Chimeric-ELISA-NBP3-08994-img0001.jpg "ELISA: DYKDDDDK Epitope Tag Antibody (M2.1) - Chimeric [NBP3-08994] - ELISA using M2.1 and Anti-i-motif DNA [iMab]. Binding curves of the rabbit IgG chimeric version of the Anti-DYKDDDDK Epitope Tag antibody M2.1 (NBP3-08994; blue line) and isotype control (NBP2-52638, anti-fluorescein; orange line) to an ELISA plate coated with Anti-i-motif DNA [iMab] at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Anti-i-motif DNA [iMab] antibody. For signal detection, a 1:4000 dilution of a Goat Anti-Rabbit IgG antibody was used.")